Calculate final volume or concentration using the M₁V₁ = M₂V₂ formula for solution dilution
Unit: mol/L (M)
Unit: mL or L (consistent)
Unit: mol/L (M)
Unit: mL or L (match V₁)
Dilution Principle: M₁V₁ = M₂V₂ (moles of solute remain constant)
The dilution calculator uses the M₁V₁ = M₂V₂ equation to determine either the final volume needed or final concentration achieved when diluting a solution. It's essential for preparing solutions with specific concentrations in chemistry labs by adding solvent to stock solutions.
M₁V₁ = M₂V₂
Find Final Volume (V₂)
V₂ = (M₁ × V₁) / M₂
Find Final Concentration (M₂)
M₂ = (M₁ × V₁) / V₂
Key Principle: The number of moles of solute remains constant during dilution (only solvent is added).
Given Information
M₁ = 5.0 M, V₁ = 10 mL, M₂ = 1.0 M
Find: Final volume (V₂)
Apply Formula
V₂ = (M₁ × V₁) / M₂
Substitute Values
V₂ = (5.0 M × 10 mL) / 1.0 M
Result
V₂ = 50 mL
Add 40 mL of solvent to 10 mL of 5.0 M solution
Inconsistent volume units
V₁ and V₂ must use the same unit (both mL or both L)
Confusing final volume with added volume
V₂ is total final volume, not volume of solvent added
Making M₂ > M₁
Dilution always decreases concentration (M₂ < M₁)
Using mass instead of concentration
Use molar concentration (M), not grams or percent
Calculate molar concentration (M = n/V)
Calculate molality (m = n/kg solvent)
Calculate equivalent concentration
Dilution is the process of decreasing the concentration of a solution by adding more solvent. The amount of solute remains constant, but the total volume increases, resulting in lower concentration.
This equation works because the number of moles of solute remains constant during dilution. Since M = n/V, then n = M × V. Before dilution: n = M₁V₁. After dilution: n = M₂V₂. Since n is constant, M₁V₁ = M₂V₂.
No, V₁ and V₂ must be in the same units (both mL or both L). However, you don't need to convert to liters - as long as both volumes use the same unit, the equation works correctly.
Serial dilution is the stepwise dilution of a solution, where each dilution uses the previous dilution as the stock solution. It's used to create very dilute solutions or a range of concentrations for experiments.
1) Calculate V₂ (final volume needed). 2) Measure V₁ (initial volume) of stock solution into a volumetric flask. 3) Add solvent until the total volume reaches V₂. 4) Mix thoroughly. Always add acid to water, never water to acid for safety.
Use the rearranged formula: M₂ = (M₁ × V₁) / V₂. This tells you the final concentration after diluting V₁ of M₁ solution to a final volume of V₂.
Yes, M₁V₁ = M₂V₂ works for any solution where concentration is expressed as molarity (M). It also works with other concentration units (%, ppm, etc.) as long as you use the same unit for C₁ and C₂.
Common ratios include 1:10 (10-fold dilution), 1:100 (100-fold), 1:1000 (1000-fold). A 1:10 dilution means 1 part stock solution + 9 parts solvent = 10 parts total (M₂ = M₁/10).
Education
Chemistry lab courses and solution preparation
Laboratory
Preparing working solutions from stock solutions
Industry
Quality control, pharmaceuticals, manufacturing
Research
Biochemistry, molecular biology, analytical chemistry